Organic Chemistry

Download e-book for iPad: A Novel Heme-Thiolate Peroxygenase AaeAPO and Its by Xiaoshi Wang

By Xiaoshi Wang

during this thesis, Xiaoshi Wang investigates the functionality and mechanism of a newly chanced on heme-thiolate peroxygenase, AaeAPO. This enzyme category comes from Agrocybe aegerita and is utilized in the conversion of inert hydrocarbons to alcohols. Xiaoshi's paintings makes a speciality of an extracellular P450 enzyme which isn't restricted in its balance and absence of solubility and hence is appropriate for common business use. the writer demonstrates that the peroxygenase catalyzes quite a lot of reactions. in certain cases the writer even describes very tricky modifications in molecules which are hugely inert. Her distinctive investigations offer a mechanistic framework for a way the peroxygenase catalyzes this type of huge variety of reactions. a tremendous spotlight of this thesis is the identity of key short-lived intermediates within the catalytic cycle of the peroxygenase, utilizing fast kinetic and spectroscopic tools, in addition to the elucidation of the thermodynamic homes of those high-energy intermediates. This paintings provides new perception into an immense category of enzymes.

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A Novel Heme-Thiolate Peroxygenase AaeAPO and Its Implications for C-H Activation Chemistry (Springer Theses) by Xiaoshi Wang


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